Rafael S Bisinotto, MVB, PhD, MS
Associate Professor
Department:
Large Animal Clinical Sciences
Business Phone:
(352) 294-4284
Business Email:
rsbisinotto@ufl.edu
About Rafael S Bisinotto
Related Links:
Accomplishments
National Milk Producers Federation Richard M. Hoyt Award
2015
·
American Dairy Science Association
Teaching Profile
Courses Taught
2020,2022-2024,2024
VEM5203 Pnt Care Clin Skill 3
2017-2025
VEM5735 Food Anml/Repro Med
2017-2025
VEM5835 Food Animal Repro Med
2018-2025
VEM5836 Adv Clk Rural Anim Md
2018-2025
VEM5220 Basic Vet Nutrition
2018-2025
VEM5504 Prevent/Productn Med
2019-2025
VEM5277 Adv Bovine Reproduct
2018-2025
VEM5352 Dairy Production Med
2018-2020,2022-2024
VME6905 Problems in Veterinary Medical Sciences
2020
VME6934 Topics in Veterinary Medical Sciences
2018,2025
VME7979 Advanced Research
2012
ANS3319C Reproductive Physiology and Endocrinology in Domestic Animals
2023
ANS6751 Physiology of Reproduction
2023
VEM5112 Animal Systems 3
2024
VEM5530 Adv Beef Practice
2025
VME6940 Supervised Teaching
Research Profile
Areas of Interest
- Biology of reproduction (embryo development, maintenance of pregnancy, and genetic control of reproduction)
- Dairy production medicine with emphasis on uterine health
- Reproductive management in dairy herds and programs for timed artificial insemination
Publications
Academic Articles
2025
Association between conditions affecting the vaginal portion of the cervix or the cranial portion of the vagina and reproductive performance in lactating dairy cows
Theriogenology.
236:82-95
[DOI] 10.1016/j.theriogenology.2025.01.024.
2025
Association of genomic predictions for fertility traits with reproductive outcomes in dairy cows under 2 estrus detection and timed artificial insemination programs
Journal of Dairy Science.
108(2):2060-2069
[DOI] 10.3168/jds.2024-25499.
2025
Associations of serum fatty acids, serum urea nitrogen, and ruminal ammonia nitrogen with residual feed intake in lactating dairy cows.
Journal of dairy science.
[DOI] 10.3168/jds.2024-25454.
[PMID] 39890064.
2025
Differences in uterine and serum metabolome associated with clinical cure failure of metritis in dairy cows.
Biology of reproduction.
[DOI] 10.1093/biolre/ioaf038.
[PMID] 39987553.
2025
Multi-omics integration and immune profiling identify possible causal networks leading to uterine microbiome dysbiosis in dairy cows that develop metritis.
Animal microbiome.
7(1)
[DOI] 10.1186/s42523-024-00366-9.
[PMID] 39789616.
2025
Targeted reproductive management for lactating Holstein cows: Economic return.
Journal of dairy science.
108(2):1584-1601
[DOI] 10.3168/jds.2024-25525.
[PMID] 39521411.
2025
Targeted reproductive management for lactating Holstein cows: Reproductive and economic outcomes of Double-Ovsynch compared with a targeted approach based on resumption of estrus.
Journal of dairy science.
[DOI] 10.3168/jds.2024-25909.
[PMID] 39986451.
2024
An artificial intelligence approach of feature engineering and ensemble methods depicts the rumen microbiome contribution to feed efficiency in dairy cows.
Animal microbiome.
6(1)
[DOI] 10.1186/s42523-024-00289-5.
[PMID] 38321581.
2024
Association between prepartum body condition score and prepartum and postpartum dry matter intake and energy balance in multiparous Holstein cows.
Journal of dairy science.
107(7):4381-4393
[DOI] 10.3168/jds.2023-24047.
[PMID] 38278298.
2024
Evaluating differences in milk production, reproductive performance, and survival associated with vaginal discharge characteristics and fever in postpartum dairy cows.
Journal of dairy science.
107(8):6079-6089
[DOI] 10.3168/jds.2023-23905.
[PMID] 38580147.
2024
Host and rumen microbiome contributions to feed efficiency traits in Holstein cows.
Journal of dairy science.
107(5):3090-3103
[DOI] 10.3168/jds.2023-23869.
[PMID] 38135048.
2024
Integrating uterine microbiome and metabolome to advance the understanding of the uterine environment in dairy cows with metritis.
Animal microbiome.
6(1)
[DOI] 10.1186/s42523-024-00314-7.
[PMID] 38802977.
2024
Investigating relationships between the host genome, rumen microbiome, and dairy cow feed efficiency using mediation analysis with structural equation modeling.
Journal of dairy science.
107(10):8193-8204
[DOI] 10.3168/jds.2024-24675.
[PMID] 38908714.
2024
Pangenomic and biochemical analyses of Helcococcus ovis reveal widespread tetracycline resistance and a novel bacterial species, Helcococcus bovis.
Frontiers in microbiology.
15
[DOI] 10.3389/fmicb.2024.1456569.
[PMID] 39318438.
2024
Revealing host genome-microbiome networks underlying feed efficiency in dairy cows.
Scientific reports.
14(1)
[DOI] 10.1038/s41598-024-77782-z.
[PMID] 39472728.
2024
Shifts in uterine microbiome associated with pregnancy outcomes at first insemination and clinical cure in dairy cows with metritis.
Scientific reports.
14(1)
[DOI] 10.1038/s41598-024-61704-0.
[PMID] 38789554.
2023
Blood metabolomics and impacted cellular mechanisms during transition into lactation in dairy cows that develop metritis
Journal of Dairy Science.
106(11):8098-8109
[DOI] 10.3168/jds.2023-23433.
2023
Differences in uterine and serum metabolome associated with metritis in dairy cows.
Journal of dairy science.
106(5):3525-3536
[DOI] 10.3168/jds.2022-22552.
[PMID] 36894419.
2023
Establishing Galleria mellonella as an invertebrate model for the emerging multi-host pathogen Helcococcus ovis.
Virulence.
14(1)
[DOI] 10.1080/21505594.2023.2186377.
[PMID] 36862000.
2023
Reproduction in the era of genomics and automation.
Reproduction, fertility, and development.
36(2):51-65
[DOI] 10.1071/RD23173.
[PMID] 38064184.
2023
Sequencing and characterization of Helcococcus ovis: a comprehensive comparative genomic analysis of virulence.
BMC genomics.
24(1)
[DOI] 10.1186/s12864-023-09581-1.
[PMID] 37648976.
2023
Targeted reproductive management for lactating Holstein cows: Reducing the reliance on exogenous reproductive hormones.
Journal of dairy science.
106(8):5788-5804
[DOI] 10.3168/jds.2022-22666.
[PMID] 37349211.
2023
Transcriptome changes associated with elongation of bovine conceptuses I: Differentially expressed transcripts in the conceptus on day 17 after insemination
Journal of Dairy Science.
106(12):9745-9762
[DOI] 10.3168/jds.2023-23398.
2023
Transcriptome changes associated with elongation of bovine conceptuses II: Differentially expressed transcripts in the endometrium on day 17 after insemination
Journal of Dairy Science.
106(12):9763-9777
[DOI] 10.3168/jds.2023-23399.
2023
Unraveling the immune and metabolic changes associated with metritis in dairy cows
Journal of Dairy Science.
106(12):9244-9259
[DOI] 10.3168/jds.2023-23289.
2022
Effects of progesterone concentrations and follicular wave during growth of the ovulatory follicle on conceptus and endometrial transcriptome in dairy cows.
Journal of dairy science.
105(1):889-903
[DOI] 10.3168/jds.2021-20193.
[PMID] 34635351.
2022
Evaluation of luteolysis, follicle size, and time to ovulation in Holstein heifers treated with two different analogs and doses of prostaglandin-F2α.
Journal of dairy science.
105(6):5506-5518
[DOI] 10.3168/jds.2021-21487.
[PMID] 35465991.
2022
Genetic analysis of fetal loss in Holstein cattle.
Journal of dairy science.
105(11):9012-9020
[DOI] 10.3168/jds.2022-22000.
[PMID] 36175228.
2022
Validation of a fully automated chemiluminescent immunoassay for cattle serum and plasma progesterone measurement.
Frontiers in veterinary science.
9
[DOI] 10.3389/fvets.2022.1064201.
[PMID] 36699340.
2021
Effects of metritis treatment strategies on health, behavior, reproductive, and productive responses of Holstein cows.
Journal of dairy science.
104(2):2056-2073
[DOI] 10.3168/jds.2020-19076.
[PMID] 33309374.
2021
Failure of clinical cure in dairy cows treated for metritis is associated with reduced productive and reproductive performance.
Journal of dairy science.
104(6):7056-7070
[DOI] 10.3168/jds.2020-19661.
[PMID] 33741169.
2021
Genes and pathways associated with pregnancy loss in dairy cattle.
Scientific reports.
11(1)
[DOI] 10.1038/s41598-021-92525-0.
[PMID] 34172762.
2020
Association between hoof lesions and fertility in lactating Jersey cows.
Journal of dairy science.
103(4):3401-3413
[DOI] 10.3168/jds.2019-17252.
[PMID] 32057429.
2020
Effect of estrous detection strategy on pregnancy outcomes of lactating Holstein cows receiving artificial insemination and embryo transfer.
Journal of dairy science.
103(7):6635-6646
[DOI] 10.3168/jds.2019-17892.
[PMID] 32418687.
2020
Effects of timing of artificial insemination and treatment of semen with a Slo3 potassium channel blocker on fertility of dairy heifers subjected to the 5-day CIDR-Synch protocol.
Journal of dairy science.
103(8):7462-7471
[DOI] 10.3168/jds.2019-18103.
[PMID] 32534924.
2020
Evaluating the association between early-lactation lying behavior and hoof lesion development in lactating Jersey cows.
Journal of dairy science.
103(11):10494-10505
[DOI] 10.3168/jds.2020-18254.
[PMID] 32981735.
2020
Impact of assisted reproduction techniques on subsequent reproductive performance of dairy heifers and lactating cows.
Theriogenology.
158:97-104
[DOI] 10.1016/j.theriogenology.2020.09.011.
[PMID] 32947065.
2020
Impact of assisted reproduction techniques on subsequent reproductive performance of dairy heifers and lactating cows.
.
158:97-104
2020
Use of on-farm milk progesterone information to predict fertility outcomes in dairy cows subjected to timed artificial insemination.
Journal of dairy science.
103(7):6600-6611
[DOI] 10.3168/jds.2019-17405.
[PMID] 32359997.
2019
Effect of presynchronization with prostaglandin F2α before the 5-d timed AI protocol on ovarian responses and pregnancy in dairy heifers.
Theriogenology.
132:138-143
[DOI] 10.1016/j.theriogenology.2019.03.019.
[PMID] 31022603.
2019
Effects of recombinant bovine interleukin-8 treatment on health, metabolism, and lactation performance in Holstein cattle II: Postpartum uterine health, ketosis, and milk production
Journal of Dairy Science.
102(11):6639-6648
[DOI] 10.3168/jds.2019-16335.
[PMID] 31495609.
2019
Genomic merit for reproductive traits. I: Estrous characteristics and fertility in Holstein heifers.
Journal of dairy science.
102(7):6624-6638
[DOI] 10.3168/jds.2018-15205.
[PMID] 31030916.
2019
Genomic merit for reproductive traits. II: Physiological responses of Holstein heifers.
Journal of dairy science.
102(7):6639-6648
[DOI] 10.3168/jds.2018-15245.
[PMID] 31030930.
2019
Haematological and biochemical profiles during the puerperium in dairy cows – Short communication.
Acta veterinaria Hungarica.
67(3):377-384
[DOI] 10.1556/004.2019.038.
[PMID] 31549539.
2018
Effects of altering the ratio of dietary n-6 to n-3 fatty acids on spontaneous luteolysis in lactating dairy cows.
Journal of dairy science.
101(11):10536-10556
[DOI] 10.3168/jds.2018-15065.
[PMID] 30197147.
2016
Biology of Preimplantation Conceptus at the Onset of Elongation in Dairy Cows.
Biology of reproduction.
94(4)
[DOI] 10.1095/biolreprod.115.134908.
[PMID] 26935601.
2016
Carryover effect of postpartum inflammatory diseases on developmental biology and fertility in lactating dairy cows.
Journal of dairy science.
99(3):2201-2220
[DOI] 10.3168/jds.2015-10337.
[PMID] 26723113.
2016
Conceptus development and transcriptome at preimplantation stages in lactating dairy cows of distinct genetic groups and estrous cyclic statuses.
Journal of dairy science.
99(6):4761-4777
[DOI] 10.3168/jds.2015-10315.
[PMID] 27016828.
2016
Effects of injectable trace mineral supplementation in lactating dairy cows with elevated somatic cell counts
Journal of Dairy Science.
99(9):7319-7329
[DOI] 10.3168/jds.2016-10989.
[PMID] 27320664.
2016
Effects of oral calcium supplementation on mineral and acid-base status, energy metabolites, and health of postpartum dairy cows.
Journal of dairy science.
99(10):8397-8416
[DOI] 10.3168/jds.2015-10527.
[PMID] 27423947.
2016
Effects of oral calcium supplementation on productive and reproductive performance in Holstein cows.
Journal of dairy science.
99(10):8417-8430
[DOI] 10.3168/jds.2015-10529.
[PMID] 27423945.
2016
Evaluation of an on-Farm culture system (Accumast) for fast identification of milk pathogens associated with clinical mastitis in dairy cows
PLoS One.
11(5)
[DOI] 10.1371/journal.pone.0155314.
[PMID] 27176216.
2016
Identification of fimbrial subunits on the genome of Trueperella pyogenes and association between serum antibodies against fimbrial proteins and uterine conditions in dairy cows
Journal of Dairy Science.
99(5):3765-3776
[DOI] 10.3168/jds.2015-10401.
[PMID] 26947291.
2016
Longitudinal metagenomic profiling of bovine milk to assess the impact of intramammary treatment using a third-generation cephalosporin
Scientific Reports.
6
[DOI] 10.1038/srep37565.
[PMID] 27874095.
2016
Mechanisms underlying reduced fertility in anovular dairy cows
Theriogenology.
86(1):254-262
[DOI] 10.1016/j.theriogenology.2016.04.038.
[PMID] 27160451.
2015
Effect of progesterone supplementation on fertility responses of lactating dairy cows with corpus luteum at the initiation of the Ovsynch protocol.
Theriogenology.
83(2):257-65
[DOI] 10.1016/j.theriogenology.2014.09.021.
[PMID] 25442385.
2015
Effects of altering the ratio of dietary n-6 to n-3 fatty acids on performance and inflammatory responses to a lipopolysaccharide challenge in lactating Holstein cows.
Journal of dairy science.
98(1):602-17
[DOI] 10.3168/jds.2014-8805.
[PMID] 25465551.
2015
Effects of intrauterine infusion of Trueperella pyogenes on endometrial mRNA expression of proinflammatory cytokines and luteolytic cascade genes and their association with luteal life span in dairy cows.
Theriogenology.
84(8):1263-72
[DOI] 10.1016/j.theriogenology.2015.07.004.
[PMID] 26234463.
2015
Meta-analysis of progesterone supplementation during timed artificial insemination programs in dairy cows.
Journal of dairy science.
98(4):2472-87
[DOI] 10.3168/jds.2014-8954.
[PMID] 25648806.
2015
Progesterone supplementation to lactating dairy cows without a corpus luteum at initiation of the Ovsynch protocol.
Journal of dairy science.
98(4):2515-28
[DOI] 10.3168/jds.2014-9058.
[PMID] 25682137.
2014
Effect of early or late resynchronization based on different methods of pregnancy diagnosis on reproductive performance of dairy cows.
Journal of dairy science.
97(8):4932-41
[DOI] 10.3168/jds.2013-7887.
[PMID] 24931532.
2014
Effect of induced subclinical hypocalcemia on physiological responses and neutrophil function in dairy cows.
Journal of dairy science.
97(2):874-87
[DOI] 10.3168/jds.2013-7408.
[PMID] 24359833.
2014
Effect of supplementing fat to pregnant nonlactating cows on colostral fatty acid profile and passive immunity of the newborn calf.
Journal of dairy science.
97(1):392-405
[DOI] 10.3168/jds.2013-7086.
[PMID] 24239079.
2014
Effects of supplemental progesterone after artificial insemination on expression of interferon-stimulated genes and fertility in dairy cows.
Journal of dairy science.
97(8):4907-21
[DOI] 10.3168/jds.2013-7802.
[PMID] 24913649.
2014
Efficacy of ampicillin trihydrate or ceftiofur hydrochloride for treatment of metritis and subsequent fertility in dairy cows.
Journal of dairy science.
97(9):5401-14
[DOI] 10.3168/jds.2013-7569.
[PMID] 24952780.
2014
Plasma anti-Müllerian hormone in adult dairy cows and associations with fertility.
Journal of dairy science.
97(11):6888-900
[DOI] 10.3168/jds.2014-7908.
[PMID] 25173464.
2014
Synchronisation of ovulation for management of reproduction in dairy cows.
Animal : an international journal of animal bioscience.
8 Suppl 1:151-9
[DOI] 10.1017/S1751731114000858.
[PMID] 24709227.
2013
Effects of 1 or 2 treatments with prostaglandin F₂α on subclinical endometritis and fertility in lactating dairy cows inseminated by timed artificial insemination.
Journal of dairy science.
96(10):6480-8
[DOI] 10.3168/jds.2013-6850.
[PMID] 23910554.
2013
Hormonal manipulations in the 5-day timed artificial insemination protocol to optimize estrous cycle synchrony and fertility in dairy heifers.
Journal of dairy science.
96(11):7054-7065
[DOI] 10.3168/jds.2013-7093.
[PMID] 24011941.
2013
Prevalence of periparturient diseases and effects on fertility of seasonally calving grazing dairy cows supplemented with concentrates.
Journal of dairy science.
96(9):5682-97
[DOI] 10.3168/jds.2012-6335.
[PMID] 23831093.
2013
Targeted progesterone supplementation improves fertility in lactating dairy cows without a corpus luteum at the initiation of the timed artificial insemination protocol.
Journal of dairy science.
96(4):2214-2225
[DOI] 10.3168/jds.2012-6038.
[PMID] 23415522.
2012
Effect of one or three timed artificial inseminations before natural service on reproductive performance of lactating dairy cows not observed for detection of estrus.
Theriogenology.
77(9):1918-27
[DOI] 10.1016/j.theriogenology.2012.01.011.
[PMID] 22444549.
2012
Effects of presynchronization and length of proestrus on fertility of grazing dairy cows subjected to a 5-day timed artificial insemination protocol.
Journal of dairy science.
95(5):2513-22
[DOI] 10.3168/jds.2011-4921.
[PMID] 22541478.
2012
Evaluation of peripartal calcium status, energetic profile, and neutrophil function in dairy cows at low or high risk of developing uterine disease.
Journal of dairy science.
95(12):7158-72
[DOI] 10.3168/jds.2012-5812.
[PMID] 23021755.
2012
Fertility in dairy cows following presynchronization and administering twice the luteolytic dose of prostaglandin F2α as one or two injections in the 5-day timed artificial insemination protocol.
Theriogenology.
78(2):273-84
[DOI] 10.1016/j.theriogenology.2012.01.012.
[PMID] 22578621.
2012
Luteal function and follicular growth following follicular aspiration during the peri-luteolysis period in Bos indicus and crossbred cattle
Reproduction in Domestic Animals.
47(2):319-327
[DOI] 10.1111/j.1439-0531.2011.01875.x.
[PMID] 21883510.
2011
Effects of gonadotropin-releasing hormone at initiation of the 5-d timed artificial insemination (AI) program and timing of induction of ovulation relative to AI on ovarian dynamics and fertility of dairy heifers.
Journal of dairy science.
94(10):4997-5004
[DOI] 10.3168/jds.2011-4240.
[PMID] 21943750.
2011
Reproductive performance of grazing dairy cows following presynchronization and resynchronization protocols.
Journal of dairy science.
94(10):4984-96
[DOI] 10.3168/jds.2011-4225.
[PMID] 21943749.
2011
The use of endocrine treatments to improve pregnancy rates in cattle.
Reproduction, fertility, and development.
24(1):258-66
[DOI] 10.1071/RD11916.
[PMID] 22394967.
2010
Applying nutrition and physiology to improve reproduction in dairy cattle.
Society of Reproduction and Fertility supplement.
67:387-403
[PMID] 21755686.
2010
Effect of interval between induction of ovulation and artificial insemination (AI) and supplemental progesterone for resynchronization on fertility of dairy cows subjected to a 5-d timed AI program.
Journal of dairy science.
93(12):5798-808
[DOI] 10.3168/jds.2010-3516.
[PMID] 21094752.
2010
Effects of feeding polyphenols from pomegranate extract on health, growth, nutrient digestion, and immunocompetence of calves.
Journal of dairy science.
93(9):4280-91
[DOI] 10.3168/jds.2010-3314.
[PMID] 20723701.
2010
Follicular wave of the ovulatory follicle and not cyclic status influences fertility of dairy cows.
Journal of dairy science.
93(8):3578-87
[DOI] 10.3168/jds.2010-3047.
[PMID] 20655426.
Grants
Jul 2024
ACTIVE
A CRISPR Approach To Determining PPARG Regulation Of Fatty Acid Availability That Influences Embryo Origins Of Conceptus Formation In Cattle
Role: Co-Project Director/Principal Investigator
Funding: US DEPT OF AG NATL INST OF FOOD AND AG
Sep 2021
ACTIVE
The origin and progression of the uterine microbiome in cattle
Role: Co-Project Director/Principal Investigator
Funding: US DEPT OF AG NATL INST OF FOOD AND AG
Mar 2021
– Mar 2022
Field Trial AlphaVision as tool for recommending insemination (Photo Data Bank)
Role: Principal Investigator
Funding: IMV TECH
Jan 2021
ACTIVE
Reproductive Biotechnology Concentration at University of Florida
Role: Faculty
Funding: US DEPT OF AG NATL INST OF FOOD AND AG
Jun 2020
ACTIVE
Impact of fertility-based selection on key physiological pathways that control reproduction in dairy cattle
Role: Principal Investigator
Funding: US DEPT OF AG NATL INST OF FOOD AND AG
Feb 2020
– May 2024
Integrating host genome and rumen microbiome to improve milk production efficiency in dairy cattle
Role: Co-Project Director/Principal Investigator
Funding: UNIV OF CALIFORNIA DAVIS
via US DEPT OF AG NATL INST OF FOOD AND AG
Jul 2019
– Sep 2020
Assessment of Efficacy of a Bovine Metritis IL-8 Immunomodulator in Multiparous Dairy Cattle Under Field Conditions
Role: Co-Investigator
Funding: ZOETIS
Jun 2019
– Jan 2020
Integrating host genome and rumen microbiome to improve milk production efficiency in dairy cattle
Role: Co-Investigator
Funding: UNIV OF ILLINOIS URBANA-CHAMPAIGN
via US DEPT OF AG NATL INST OF FOOD AND AG
Feb 2019
– Aug 2020
Comparison of reproductive performance of dairy cattle following a multivalent modified live postpartum vaccine protocol or multivalent killed pre partum vaccine protocol
Role: Principal Investigator
Funding: ELANCO ANIMAL HEALTH
Nov 2018
– Jun 2022
Test of a new superovulatory drug (compound X by Merck Animal Health) for cattle
Role: Co-Project Director/Principal Investigator
Funding: MERCK ANIMAL HEALTH
Oct 2018
– Oct 2019
Strategies to extend sperm cell lifespan and improve fertility outcomes in dairy heifers
Role: Principal Investigator
Funding: IMV TECH
Jun 2018
– Jun 2021
Evaluation of proestrus characteristics, hormonal profile, and interval to ovulation in Holstein heifers treated with different formulations of prostaglandin F2a
Role: Principal Investigator
Funding: MERCK ANIMAL HEALTH
May 2017
– Dec 2019
A possible role for interleukin 8 during adaptation to lactation
Role: Principal Investigator
Funding: CORNELL UNIV
via US DEPT OF AG NATL INST OF FOOD AND AG
Education
Postdoctoral Associate
2015
·
Cornell University
PhD – Animal Sciences
2014
·
University of Florida
MS – Animal Molecular and Cellular Biology
2010
·
University of Florida
DVM
2007
·
University of São Paulo
Contact Details
Phones:
- Business:
- (352) 294-4284
Emails:
- Business:
- rsbisinotto@ufl.edu
Addresses:
- Business Mailing:
-
PO Box 100136
GAINESVILLE FL 32610 - Business Street:
-
2250 SHEALY DR
GAINESVILLE FL 32611
